R5 ELISA Test Results for Gluten-Containing Products Testing “Low Gluten” & “High Gluten” Using the Nima Sensor

Bottom Line: Based on testing done to date by Gluten Free Watchdog, products testing “low gluten” using Nima tested from below the limit of detection of 1 ppm gluten up to 18,963 ppm gluten using the R5 ELISA. Barley flour also tested low gluten. Based on these results it is difficult to assess the meaning or understand the usefulness of a “low gluten” Nima reading.

Why did we test gluten-containing products: We tested a variety of gluten-containing products out of […]

Products testing low gluten using the Nima Sensor test less than 5 ppm using the R5 ELISA: False Positives?

Bottom Line: In some cases packaged food labeled gluten-free (or appearing to be free of gluten containing ingredients) testing “low gluten” using the Nima may test below the lower limit of quantification of 5 parts per million of gluten (and below the limit of detection of 1 ppm) using the fully validated R5 ELISA Mendez Method (Ridascreen Gliadin R7001).

Comparison Testing: Results comparing the Nima to the R5 ELISA included in this summary report are for the same product. In […]

Mercury and the Gluten-Free Diet

Bottom line: Rice sent by Gluten Free Watchdog to the Trace Element Analysis Core Laboratory at Dartmouth College contained very small amounts of total mercury.
What is causing the concern about mercury and the gluten-free diet? Two recently published studies have caused concern about the gluten-free diet and mercury. The latest study “Unintended Consequences of a Gluten-Free Diet” published in the journal Epidemiology by Bulka CM et al, found higher levels of blood total mercury in people self-reporting following a gluten-free diet as […]

Troubling Gluten Testing Data Released by Nima: But Hold the Phone

Yesterday the Nima Sensor team released testing data that has caused me a great deal of concern. They are reporting that 24% of packaged foods tested by consumers and uploaded to the Nima app have tested gluten detected. They are also reporting in a new graphic that it is very rare for the Nima to test positive for gluten at levels below 20 parts per million.

BUT this is not the complete story. The Nima is NOT a fully validated […]

Gluten Free Watchdog Updated Position Statement on Oats

Background: Two scientific publications from PepsiCo scientists provide us with the best data to date on the state of contamination of oats sold as gluten-free in the US (1, 2). Extensive testing by PepsiCo found 1 in every 57 servings tested (0.25 g sample taken from each 45 to 50 g ground serving) to contain gluten at levels at or above 20 parts per million using the R5 ELISA R7001 assay. The scientists point out that the number of […]

Lateral Flow Devices (Nima, Gluten Tox Home, EZ Gluten) to test food for gluten: Manufacturer & Consumer Use

This article was originally published in 2012 on Due to the marketing of the new consumer lateral flow device (LFD) NIMA there is a renewed interest in LFDs. An updated version of the article is republished here. If you are a manufacturer or consumer who currently uses or is thinking of using a LFD please take the time to read this article.

 Thank you to Thomas Grace of Bia Diagnostics, and Adrian Rogers of Romer Labs for sharing their […]

Grocery stores: please do not place gluten-free flour below wheat flour on store shelves

NOTE: A pdf of the letter is available at this link GFWDStoreLetter

Thank you to the Gluten Free Watchdog subscriber for sharing the attached photo.

Dear manager,

It has recently come to our attention at Gluten Free Watchdog, LLC that gluten-free foods are being placed below wheat-based foods on store shelves. This may not seem like much of an issue. However, when wheat-based food “leaks” as is often the case with bags of flour it is a health hazard. Wheat flour ends up […]

Using Nima to test mustard for gluten contamination

The video is embedded below. First a recap of Nima testing to date …

Sampling methodology for Nima: Gluten Free Watchdog has been assessing Nima’s sampling methodology. Users are advised to place a small sample of food inside a disposable capsule. A small sample is defined as “pea sized.”

In the opinion of Gluten Free Watchdog, this sampling method is not well suited for testing packaged foods that may have heterogeneous (unevenly distributed) gluten contamination or restaurant meals with spotty cross […]